Fig 4. Shear stress caused a polarized Rac activation in BAECs after pre-treatment of ML-7 and CytoD but eliminated by NOCO.

The images of BAECs transfected with biosensor (left panel) pretreated with ML-7(A, n = 7), CytoD(B, n = 6) or NOCO(C, n = 8) upon laminar flow application were dealt by the imaging analysis(right panel). The 3D spatiotemporal map (D) of averaged single cell images showed an obvious shear stress-induced Rac activation at the downstream of BAECs from ML-7 (Above) and CytoD group (Middle), but not NOCO (Below). After that, the data of the thirtieth minute from three groups were fitted by logistic curve. Above was the ML-7 group, middle was CytoD and below was the NOCO group (E). The arrow indicated the main flow direction.