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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Exp Zool A Ecol Integr Physiol. 2017 Jun;327(5):262–272. doi: 10.1002/jez.2086

Figure 1.

Figure 1

Timeline of experimental methods. At 12 days post-hatch, young were assigned to either an elevated corticosterone (CORT) treatment group or a control group. Dosing with CORT or the control vehicle continued until day 28 post-hatch. On day 14, we collected the first blood sample to quantify corticosterone concentrations and bacteria killing ability. We also measured bacteria killing ability from blood samples collected on days 36 and 60. On days 60 and 100, one-half of the individuals were challenged with lipopolysaccharide (LPS). We collected a blood sample before challenge to measure baseline CORT and a second blood sample one hour after challenge to measure LPS-induced CORT concentrations. We also collected blood samples on days 68 and 108 to quantify LPS-specific antibody (Ab) titers. Finally, on day 100 we collected a blood sample before LPS challenge to quantify baseline haptoglobin (Hp) concentrations and collected a second blood sample 12 hours later to quantify Hp concentrations in response to LPS.