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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2017 Nov 15;43:5A.11.1–5A.11.14. doi: 10.1002/cpsc.37

Figure 2.

Figure 2

The RhAAVS1 locus (bottom) is located in the first intron of the PPP1R12C gene. The rhesus AAVS1-CAG-human truncated copGFP donor plasmid (top) was modified from the human AAVS1-CAG-copGFP vector by replacing the human AAVS1 homology arms with orthologous rhesus AAVS1. The Southern Blot probe is found in the left homology arm (red bar) while the forward and reverse PCR primers used for detecting targeted integration of the donor plasmid in RhiPSCs are located outside of the donor plasmid and within the SA-2A-puro gene of the plasmid respectively (blue arrows).