Table 1.
Locations of the Top 10 Potential Off-Target Sites
| Off-target Sequence | PAM | Chr | Position | Strand | MM Locations | # of MMs | Score | Overlapping Genea | |
|---|---|---|---|---|---|---|---|---|---|
| 1 | AGGGCCACTTGGGACAGGAC | TAG | 4 | 77699284 | − | 1,10 | 2 | 7.414830508 | None |
| 2 | GGCCCCACTAGGGACAGGAC | AAG | 3 | 160355267 | + | 3,4 | 2 | 5.146703297 | CADPS2b |
| 3 | GGGGCCAGTGGGGACAGGAC | AGG | 13 | 127282774 | − | 8,10 | 2 | 5.028448276 | None |
| 4 | GGCCCCATTAGGGACAGGAC | CAG | 1 | 18536016 | + | 3,4,8 | 3 | 2.448888889 | None |
| 5 | GGGACCATGAGGGACAGGAC | TGG | 19 | 19145046 | − | 4,8,9 | 3 | 1.51751634 | ENSMMUG00000003164b |
| 6 | GGGGGCAGCAGGGACAGGAC | TGG | 17 | 93563191 | − | 5,8,9 | 3 | 1.482630907 | None |
| 7 | GGAGCTAATAGGGACAGGAC | TGG | 14 | 30064062 | − | 3,6,8 | 3 | 1.481577778 | None |
| 8 | GGGAGCACTAAGGACAGGAC | CGG | 7 | 46978018 | − | 4,5,11 | 3 | 1.446502058 | ITGA11b |
| 9 | GGGAGCAGTGGGGACAGGAC | TGG | 11 | 3181345 | − | 4,5,8,10 | 4 | 1.257112069 | None |
| 10 | GGGGCCAGTGGGGACAGGAA | GGG | 12 | 95806523 | − | 8,10,20 | 3 | 1.141953521 | None |
MM stands for Mismatch.
a
Gene information was found using BLAST online analysis (www.ensembl.org).
b
Off-target sequence is located in the intron of the corresponding gene.