. Author manuscript; available in PMC: 2018 Nov 15.

Published in final edited form as: Curr Protoc Stem Cell Biol. 2017 Nov 15;43:5A.11.1–5A.11.14. doi: 10.1002/cpsc.37

Table 2.

Primers and probes used in this protocol.

Assay	Site	Forward Primer (5′-3′)	Reverse Primer (5′-3′)
Southern Blot	Rhesus AAVS1 5′ homology arm	tatgaccatgattacgccgccacctccttcaggttccagcttcct	tatgctatacgaagttatgcctgtccctagtggccccacggtggg
qPCR	Puromucin N-acetyl-transferase	gcaacctccccttctacgag	cgggcttgcgggtcatg
NHEJ PCR	Rhesus AAVS1 WT Allele	tgctttctttgcctggacac	tgatgcacagaggaacagtac
Targeted integration PCR		tcctgctttcactgacctgc	ggcttgtactcggtcatcctag
Off-target analysis	OT-1	agagagtgtgtaggtgggtatt	gcctcttggaatggtgctataa
	OT-2	aaagttcaaagtggagacagga	aagagcaagcagggtgttta
	OT-3	ccaggttagaggaggcataaac	ggaactgaagagatgcccaa
	OT-4	tttgaaccgtgctcctagc	gctggacttcaggcctattt
	OT-5	gctcatcttgagagagagaagaaa	gctgtagcgggttatttgaatg
	OT-6	atgtcacaagcgtgcctt	atccatgaagcctccaagatg
	OT-7	ttctggtggttacacttcattca	agggctctttgacagatcattac
	OT-8	ggttactgttcttagccactgt	gactcagggaatgcgtttct
	OT-9	tgtgaggtactgtgcctgga	gaaaggccattgcattagga
	OT-10	tccacctctcaggttcaagc	acccaaggaacctttgcttt