| 1 cycle: | 94°C for 5 min |
| 35 cycles: | 94°C for 30 sec |
| 57°C for 30 sec | |
| 68°C for 1 min | |
| 1 cycle: | 68°C for 5 min |
Note: OT10 PCR reaction requires an annealing temperature of 61°C.
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