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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2017 Jul 20;409(24):5645–5654. doi: 10.1007/s00216-017-0500-x

Figure 1.

Figure 1

SDS-PAGE analysis of cusativin enriched at different stages of purification from seed extracts.

Lanes 1, seed extract; 2, flow-through of Sephadex G-25; 3, CM-Cellulose column fraction eluted with 200 mM NaCl; 4, CM-cellulose fraction purified by Sephadex G-75 column; and 5, polypeptide size standards. About 20–25 μL of protein sample from each step of purification was concentrated to 10 μL, boiled with equal volume of Laemmli buffer and analyzed on a 4–21% polyacrylamide gradient gel. Position of ~23 kDa polypeptide is indicated by an arrow.