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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2017 Jul 20;409(24):5645–5654. doi: 10.1007/s00216-017-0500-x

Figure 3.

Figure 3

LC-MS/MS analysis of trypsin digest of RNase cusativin.

(A) Total ion chromatogram (TIC) of all the observed peptide ions. (B) Extracted ion chromatogram (XIC) for m/z 561.8331 (3.5 ppm), corresponding to tryptic peptide HGIDLLSVLR. (C) Mass spectrum associated with the XIC at 21.3 min. (D) Tandem mass spectrum (MS/MS) of the precursor ion, m/z 561.8, following collision-induced dissociation (CID) in the ion trap. The observed sequence informative product ion series, bn (sharing common N-terminal end) and yn (with common C-terminal end), with a subscript denoting the position of cleavage on peptide backbone, are labeled and plotted. (E) Predicted amino acid sequence of the polypeptide following cucumber proteome database search analysis with the tandem mass spectrometry data. The observed tryptic peptides are shown bold type and underlined.