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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2017 Jul 20;409(24):5645–5654. doi: 10.1007/s00216-017-0500-x

Figure 4.

Figure 4

LC-MS/MS analysis of recombinant cusativin digestion product, AGA[Cm]U[Gm]AA[yW]A[Ψ][m5C]>p from yeast tRNA(Phe).

(A) Total ion chromatogram (TIC). (B) Extracted ion chromatogram (XIC) for m/z 1389.7, corresponding to AGA[Cm]U[Gm]AA[yW]A[Ψ][m5C]>p (position 29–40). (C) Mass spectrum of the XIC at 40.5 min. Note the presence of triply deprotonated anions in the spectrum The spectrum also shows the presence of oligonucleotide anion corresponding to the loss of [yW] base (m/z 1264.1). (D) Tandem mass spectrum (MS/MS) of the precursor ion, m/z 1389.7, following collision-induced dissociation (CID). The observed sequence informative product ion series, cn (sharing common 5′ endare indicated by *) and yn (sharing common 3′ end and indicated by ◆) with a subscript denoting the position of cleavage on phosphodiester backbone are labeled and plotted.