Figure 6.

PGC-1α appears to be required for KISS1-mediated induction of beta-oxidation-related gene expression and AMPK-mediated phosphorylation of ACC. A PCR Array was used to probe expressions of various genes in C1861.9^Vector (V), C8161.9^KFM59 (KFM), and C8161.9^{KFM59/shPGC-1α} (K/shP) cells. Selected upregulated genes are shown in (a & b). (c) mRNA expression of beta-oxidation-related genes, ACC1, ACC2, ACLY, and PPARα in C1861.9^Vector, C8161.9^KFM59, and C8161.9^{KFM59/shPGC-1α#1}(K/shP1), and C8161.9^{KFM59/shPGC-1α#2} (K/shP2)cells were quantified by qRT-PCR. (d) PGC-1α, phos-ACC at serine 79, ACC, phos-AMPKβ at serine 108, AMPKβ, phos-AMPKα at threonine 172, AMPKα, and KISS1 proteins from C1861.9^Vector, C8161.9^KFM59, C8161.9^KFMΔSS6(ΔSS), C8161.9^{KFM59/shPGC-1α#1} and C8161.9^{KFM59/shPGC-1α#2} whole cell lysates were quantified using immunoblot analysis with beta-actin as loading control. (a-c) N ≥ 3; error bars, SEM; * P , <0.05