Skip to main content
. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Mol Med (Berl). 2017 Jun 8;95(9):951–963. doi: 10.1007/s00109-017-1552-2

Figure 7.

Figure 7

PGC-1α knockdown reverses KISS1-mediated metastasis suppression in vivo. (a) Representative images of lung metastasis after i.v. injection of C1861.9Vector (V), C8161.9KFM59 (KFM), C8161.9KFM59/shPGC-1α#1 (K/shP1), and C8161.9KFM59/shPGC-1α#2 (K/shP2) cells. The lung metastases were visualized macroscopically and using green fluorescent protein, lung metastases were quantified (b). N =10; error bars, SEM; * P , <0.05 (c) Representative images of spontaneous metastasis following orthotopic tumor growth of C1861.9Vector, C8161.9KFM59, C8161.9KFM59/shPGC-1α#1 and C8161.9KFM59/shPGC-1α#2 cells and metastases were illuminated using GFP. (d) Incidence of spontaneous metastases to distant organs. (e)Orthotopic tumor volume was measured at 2, 3, 4, and 5 weeks. n = 9-10; error bars, SEM; * P , <0.05