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. 2017 Aug 7;36(48):6668–6679. doi: 10.1038/onc.2017.278

Figure 1.

Figure 1

MFTF stimulates JB6 P+ cell transformation. SKH-1 mice (n=5/group) were fed either a HFD or LFD for 4 weeks. Visceral (parametrial and epididymal) adipose tissue was removed to make a filtered conditioned medium (MFTF). (a) Percentage of clones growing in soft agar (% colony formation) significantly increases in JB6 P+ cells cultured with MFTF compared no treatment (control; Cont). No significant change in the percentage of colony formation in soft agar is observed in JB6 P− cells cultured with MFTF. (b) JB6 P+ colonies growing in soft agar with HFD MFTF is significantly inhibited with proteinase K, but not with lipase, RNase A or DNase A. (c) JB6 P+ colonies growing in soft agar decrease as MFTF is exposed to increasing temperatures for 30 min prior to treating the cells in agar. (d) Protein Profiler angiogenesis array of fat tissue filtrates of LFD-fed mice (top panel) and HFD-fed mice (bottom panel). HFD selectively upregulated protein levels of several key adipokines, hormones and growth factors in MFTF, versus those seen in MFTF of LFD-fed mice (boxed proteins). Dot intensity was analyzed by ‘Image J’ software. Data are labeled as the percent of the control (reference) dots located in the upper left hand corner of the arrays. Data are presented as mean±s.d. of values from triplicate. Statistical significance was determined using a one-way ANOVA (**P<0.01, ***P<0.001).