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. 2017 Aug 7;36(48):6668–6679. doi: 10.1038/onc.2017.278

Figure 5.

Figure 5

Transforming activity of HuFTF is associated with FGF2 levels and is dependent on FGFR-1. Fat tissue filtrates were made from human visceral adipose tissue (HuFTF) from four donors undergoing hysterectomy. (a) Percentage of clones growing in soft agar (% colony formation) significantly increases in JB6 P+ and NMuMG cells cultured with HuFTF from donors 1, 2 and 4 compared no treatment (control; Cont). (b) HuFTF with higher concentrations of FGF2 are more potent at stimulating cell transformation. (c) Immunodepletion of FGF2 in HuFTF significantly attenuates JB6 P+ colony formation in soft agar. (d) JB6 P+ cells deficient in FGFR-1 fail to grow in soft agar above baseline when cultured with FGF2 and HuFTF in soft agar. Percentage of clones growing in soft agar (% colony formation) significantly increases in JB6 P+ cells deficient in FGFR-1 cultured with HGF compared no treatment (Untx). Data are presented as mean±s.d. of values from triplicates and statistical significance was determined using a one-way ANOVA (*P<0.05, **P<0.001, ***P<0.0001).