A, the pH‐mediated potentiation of the sustained current (I
S) was calculated as I
S(post) – I
S(pre)/I
S(pre) × 100. The measurements for pre (control solution) and post (test solution) currents were acquired as the average current amplitude obtained during the last second of the test pulse (shown in red). B, ASIC current trace of an L‐cell expressing ASIC2b following application of an external solution of pH 6.0 (black trace, left) or 5.0 (black trace, right). After washout, the cell was preincubated in a solution (pH 7.4) containing 10 μm E‐2. Thereafter, the cell was exposed to an external solution (pH 5.0) and E‐2 (10 μm, red trace). C, ASIC1a current trace following exposure to a control solution (pH 6.0, black) and a solution containing 10 μm E‐2 (red) of an L‐cell transfected with ASIC1a cDNA. [Color figure can be viewed at wileyonlinelibrary.com]