Figure 8. Heteromeric glycine receptors mediate synaptic glycine currents in PV+ INs.

A, glycinergic mIPSCs captured from PV+ INs (overlaid traces) were used to undertake peak‐scaled non‐stationary noise analysis and subsequently estimate mean single channel conductance of synaptically located glycine receptors (right plot). PV+ INs exhibited relatively low conductances, which is consistent with the presence of heteromeric α/β glycine receptors. B, continuous glycinergic mIPSC recordings prior to (control) and during picrotoxin application (left) and overlaid mIPSCs captured under each condition. Inset (bottom right) compares averaged mIPSCs recorded in control and picrotoxin (traces offset for comparison). C, bar plots compare group data for mIPSC frequency, amplitude, rise time and decay time constant under control conditions (C) and in picrotoxin (P). Values for individual neurons are shown as filled circles. Picrotoxin did not alter mean mIPSC properties, thus confirming these currents are mediated by heteromeric α/β GlyRs in PV+ INs.