Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 30;7:16665. doi: 10.1038/s41598-017-16853-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

KF inhibits RANKL-induced osteoclast formation in vitro. (A) BMMs were treated with various concentrations of KF followed by 30 ng/ml M-CSF and 50 ng/ml RANKL, after incubation for 7 days, cells were fixed with 4% paraformaldehyde and subjected to TRAP staining. (B) Number of TRAP-positive multinucleated osteoclasts. (C) KF shows no cytotoxicity at low concentrations. Viability of KF treated BMM cells after being incubated for 48 h, 72 h and 96 h. (D) The half-maximal inhibitory concentration (IC50) of kaempferide was 159.8 ± 15.6 μM, 90.72 ± 10.3 μM and 43.13 ± 8.7 μM, respectively. (E) KF suppresses RANKL-induced gene expression. BMM cells were cultured with M-CSF (30 ng/ml), RANKL (50 ng/ml) and KF (6.25 μM and 12.5 μM) for 5 days. RANKL-inducible gene expression was analyzed by real time PCR. RNA levels were normalized relative to the expression of Beta-actin (*p < 0.05, **p < 0.01).