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. 2017 Nov 30;7:16646. doi: 10.1038/s41598-017-16816-1

Figure 3.

Figure 3

Ex vivo activation of HIV-1 expression by RVX-208 and PFI-1. (A) Resting CD4+ T cells were isolated from virally suppressed HIV-1-infected patients and pulse-treated with prostratin (1 μM), SAHA (0.5 μM), RVX-208 (50 μM) or PFI-1 (5 μM) for 18 hours. Cell-associated total RNA was extracted, HIV-1 RNA levels were quantified using RT-qPCR for the Poly A region, and fold increase was determined relative to DMSO control. *p < 0.05, **p < 0.01. (B) Resting CD4+ T cells isolated from another two HIV-1 patients were treated with PHA (5 μg/ml), RVX-208 (50 μM) or PFI-1 (5 μM) and viral RNA was quantified in cell culture supernatant 18 hours after the addition of drugs. Results are depicted as fold increase in viral RNA relative to control cultures.