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. 2017 Nov 30;7:16646. doi: 10.1038/s41598-017-16816-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RVX-208 and PFI-1 do not up-modulate the expression of CD4, CXCR4 and CCR5 of CD4+ T cells. (A and B) CD4+ T cells isolated from healthy donors were treated with prostratin (1 μM), RVX-208 (50 μM) or PFI-1 (5 μM) and stained for surface markers 72 hours after the treatment initiation. Fractions of CD4, CXCR4 (A) and CCR5 (B) positive cells were analyzed by flow cytometry. (C) The percentage of CD4, CXCR4 and CCR5 positive cells was calculated. *p < 0.05.