Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 30;7:16624. doi: 10.1038/s41598-017-16998-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Agarose gel electrophoresis of colony PCR used to confirm editing at multiple loci. (A) A lacZ gene knockout with a 1018bp deletion: lanes 11 and 23 are control group samples from the original strain, lane 12 is the marker, and the other lanes are experimental group samples from edited strains. Colony PCR products from the edited lacZ gene are 982bp, and that of the original is 2000bp long. (B) A poxB gene knockout with 443bp deletion: lanes 11 and 22 are control group samples, lane 23 is the marker, and the other lanes are experimental group samples. Colony PCR products from edited poxB gene are 1083bp, and that of the original is 1520bp long. (C) 1018bp of lac Z replaced by a 915bp kan fragment: lanes 11 and 23 are control group samples, lane 12 is the marker, and the other lanes are experimental group samples. Colony PCR products of the edited lacZ gene are 1897bp, and that of the original is 2000bp long. (D) 443bp of poxB replaced by a 915bp kan fragment: lanes 10 and 21 are control group samples, lane 11 is the marker, and the other lanes are experimental group samples. Colony PCR products from the edited poxB gene are 1998bp, and that of the original is 1520bp long. Lane 1 had a larger PCR product, indicated one unsuccessful editing event.