Table 2.

Efficiency of two rounds of consecutive editing using CAGO.

Editing type	First locus editing			Second locus editing
	First-round recombination efficiency (CFU/µg DNA)	Positive colonies/Tested colonies of the first-round recombination	Positive colonies/Tested colonies (%) of the CAGO editing	First-round recombination efficiency (CFU/µg DNA)	Positive colonies/Tested colonies of the first-round recombination	Positive colonies/Tested colonies (%) of the CAGO editing
Genomic deletion	198.3 ± 17.6	5/5	ΔlacZ (40/40) (100%)	343.7 ± 40.5	5/5	ΔpoxB (39/40) (95%)
Genomic replacement	251.3 ± 51.2	4/5	lacZ::kan (39/40) (95%)	382.7 ± 28.3	5/5	poxB::kan (39/40) (95%)