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. 2017 Nov 30;7:16658. doi: 10.1038/s41598-017-16933-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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One frame of 515-ms duration, high-speed acquisition from an E16.5 embryo with flow in the liver (L) and umbilical cord (UC) showing (a) B-mode image, (b) non-stationary flow speckle, (c) Doppler spectrogram in the region of the liver using the averaged slow-time data from the boxed region in (a), d) color-flow Doppler image. The yellow line in (c) 258 ms after the start of the spectrogram, indicates the slow-time point of the images. The full image sequence is available as Movie 1.