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. 2017 Nov 30;7:16674. doi: 10.1038/s41598-017-16851-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Yeast two-hybrid analysis for interactions between the SlER-WRKYs and other key ripening-related transcription factors. (a) The coding regions of SlWRKY16/17, SlRIN, and SlERF2b/7 were cloned into the pGBKT7 vector to create the DBD-constructs. The coding regions of the SlER-WRKYs were cloned into the pGADT7 vector to create the AD-constructs. (b) Yeast two-hybrid analysis for the interaction between SlWRKY16 and 17 and for the interactions between SlRIN, SlERF2b or SlERF7 and SlWRKY17, 33, 53 or 54. The Y2H strain harboring the indicated plasmid combinations was grown on either the SD/-Leu/-Trp nonselective media (DDO), SD/-Leu/Trp/-His/-Ade/AbA selective media (QDO) or QDO followed by X-gal staining (QDO + X-α-gal). Control tests for each assay were the transformants of the pGBKT7-SlWRKY16/SlWRKY17/SlRIN/SlERF2B/SlERF7 with empty pGADT7 vectors (A). The transformants with pGBKT7–53 and pGADT7-T as well as pGBKT7-Lam and pGADT7-T served as positive and negative controls, respectively.