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. 2017 Nov 30;7:16650. doi: 10.1038/s41598-017-16932-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR/Cas9 works efficiently in naïve human embryonic stem cells. Editing efficiencies for TUNA, EMX1 and MEG3 after transfecting pX335, pX330-sgRNA1 or pX330-sgRNA2 plasmids in the naïve hESCs. As a control, mock-treated naïve hESCs (no plasmid) were used. Grey dots represent two biological replicates, the asterisk indicates the average of both replicates.