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. 2017 Nov 30;7:16616. doi: 10.1038/s41598-017-16882-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ISG20 and tetherin restrict HBV spread in HepG2-NTCP cells. (A) Schematic of experimental design. (B) Co-culture of HBV donor cells (HepDE19) with acceptor cells (HepG2-NTCP stably overexpressing ISG20 or tetherin or empty/control) at a 1:10 ratio. PEG was added in the co-culture for an additional 10 days. High content imaging analysis of HBcAg positive cells was performed using ImageXpress. Data from five biological replicates are shown as means ± s.d. (one-way ANOVA, *P < 0.05; **P < 0.001). As 100% spread we set the spread in HepG2-NTCP-empty cells. (C) Schematic representation of restriction of HBV spread in ISG20 or tetherin expressing cells.