Table 1.

HBV infection optimization using 100 GEQ/cell inoculum.

	HBeAg NCU/ml	Fold change
Effect of DMSO and spinoculation during initial infection
Untreated	1.02	1
+2% DMSO	4.24	4
+2% DMSO + spinoculation	6.21	6
Effect of duration of infection
3 dpi	1.03	1
5 dpi	6.14	6
7 dpi	4.24	4
Effect of seeding density (cells/well)
5,000	0.13	1
10,000	0.42	3
30,000	4.24	33

Data represent average of five biological replicates.