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. 2017 Nov 30;7:16623. doi: 10.1038/s41598-017-16811-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative positive ion mode MALDI-TOF-MS spectra of released and derivatized N-glycans. Exemplary mass spectra of (A) Pancreatic duct cell line hTERT-HPNE, (B) Pancreatic duct adenocarcinoma (PDAC) sister cell lines PaTu-S and (C) PaTu-T, as well as the primary PDAC cell cultures (D) PDAC1, and (E) PDAC2 are shown. Major glycan peaks are annotated. Symbolic glycan depictions represent compositions and the presence of structural isomers cannot be excluded. Glc = glucose; Gal = galactose; Man = mannose; GlcNAc = N-acetylglucosamine; GalNAc = N-acetylgalactosamine; Fuc = deoxyhexose, fucose; NeuAc = N-acetylneuraminic acid; *reducing end adduct.