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. 2017 Oct 30;21(6):625–632. doi: 10.4196/kjpp.2017.21.6.625

Fig. 6. Reduced cell viability and DJ-1 down-regulation in the A53T synuclein overexpressed SH-SY5Y cells.

Fig. 6

(A) SH-SY5Y cells were infected with A53T synuclein lentivirus for 72 h and the cells were treated with various concentration of 6-OHDA for 24 h. The expression level of synuclein was examined by immunoblotting (upper panel). Then, cell viability was examined by MTT assay. (B) SH-SY5Y cells were infected with 1, 5, or 10 m.o.i. of A53T synuclein lentivirus for 72 h and the cell lysates were electrophoresed and immunoblotted with indicated antibody. The intensity of each band was normalized to that of β-actin and presented in bar graphs. (C) SH-SY5Y cells were transfected with control or DJ-1 siRNA for 48 h. Then, cell viability was examined by MTT assay cells and DJ-1 expression was examined by immunoblotting. The values represent the means±SD (n=4). **p<0.01 vs. Control. ***p<0.001 vs. Control.