Fig. 7.

TRPA1 blockade and antioxidant reduced the number of fluorescent macrophages accumulated at the site of pSNL. a In vivo imaging and quantitative data (NIR area/total ROI) of NIR labeled macrophages (at day 10 after surgery) in sham/pSNL mice at baseline (BL), 1 and 3 h after HC-030031 (HC03, 100 mg kg⁻¹, i.p.) (n = 4, ***P < 0.001 pSNL HC03 vs. pSNL Veh HC03; two-way ANOVA followed by Bonferroni post hoc analyses). b Representative images and F4/80⁺-cell number surrounding the injured nerve trunk (at day 10 after surgery) of sham/pSNL-mice at BL and 1, 3 and 6 h after HC03 or alpha-lipoic acid (αLA) (both, 100 mg kg⁻¹, i.p.). (n = 4, ***P < 0.001 pSNL vs sham; ^§§§ P < 0.001 pSNL HC03, αLA vs. pSNL veh; one-way ANOVA followed by Bonferroni post hoc analyses) (Scale bars, 200 µm; inside (in) and outside (out) sciatic nerves). Data are represented as mean ± s.e.m