Figure 7.

Intraperitoneal mineralo-organic particles promote pro-inflammatory activities in C57BL/6 mice. (A) DMEM without (“−”) or with mineralo-organic particles (“P”) were injected into the peritoneum of C57BL/6 mice. Eight hours after injection, intraperitoneal lavage was performed on the treated animals to access intraperitoneal cell population profiles. Data are representative of three independent experiments. Scale bars: 10 μm. (B) Mineralo-organic particles were intraperitoneally injected into C57BL/6 mice either together with anti-HMGB1-neutralizing antibodies (“Anti-HMGB1”) or isotype control antibody of chicken IgY (“Control IgY”). A vehicle control without the addition of mineralo-organic particles and antibodies also was included in this study. Two hours after injection of mineralo-organic particles, the RAW264.7 cells previously primed with IFN-γ were intraperitoneally injected into the mice. The inflammatory response elicited after six hours post-injection of mineralo-organic particles was assessed by detecting TNF-α via ELISA. Data are shown as means ± SEM and the results of at least three independent experiments. **p < 0.005, vs. vehicle control. ^# p < 0.05, vs. Control IgY. (C) Particles were intraperitoneally injected into C57BL/6 mice alone or together with GW311616, followed by injection of interferon (IFN)-γ-primed RAW264.7 cells two hours later. The inflammatory response elicited after 6-hour post-injection of particles was assessed by ELISA. Data are shown as means ± SEM and the results of at least three independent experiments. *p < 0.05 and **p < 0.001, vs. vehicle control. ^## p < 0.005, vs. GW311616-free control.