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. 2017 Nov 30;7:16675. doi: 10.1038/s41598-017-16856-7

Figure 3.

Figure 3

The gene expression profiles of hepatocyte-specific genes and drug metabolic-associated genes. MRC5 cells were transduced with LV-5TFs for 12 hr, and cultured until day 28. (A) The hepatic gene (ALB and AAT) and fetal-specific hepatic gene (TTR and AFP) expression levels were measured by real-time RT-PCR. The gene expression levels in PHH 48 hr were taken as 1.0. *p < 0.05; **p < 0.01 (vs hiHeps). (B) The CYP enzyme gene (CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) expression levels were measured by real-time RT-PCR. The gene expression levels in PHH 48 hr were taken as 1.0. *p < 0.05; **p < 0.01 (vs hiHeps). (C) The gene expression levels of Na+-taurocholate cotransporting polypeptide (NTCP) and uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) were measured by real-time RT-PCR. The gene expression levels in PHH 48 hr were taken as 1.0. *p < 0.05; **p < 0.01 (vs hiHeps). (D) The hepatic transcription factor (FOXA2 and HNF4A) expression levels were measured by real-time RT-PCR. The gene expression levels in PHH 48 hr were taken as 1.0. *p < 0.05; **p < 0.01 (vs hiHeps). All data are represented as means ± SD (n = 3). PHH 48 hr: PHH cultured for 48 hr after plating; PHH 0 hr: PHH collected immediately after thawing.