Figure 3.

EHV1 preferentially infects the basolateral surface of EREC and disruption of ICJ overcomes the restriction to EHV1 infection at the apical surface. (a) To compare EREC susceptibility to EHV1, cells were exposed at either the apical surface or basolateral surface to EHV1 (MOI 1). Cells were fixed in methanol 10 hpi and stained for IEP. The total number of plaques was counted in five different fields of approximately 3∙10⁴ cells for each condition (left). Average plaque latitudes were measured on 10 individual plaques (right). Experiments were performed in triplicate on primary EREC of 3 different horses. Data are represented as means + SD and significant (P < 0.05) differences for 03P37 strain infection are indicated by lower case letters and for the 97P70 strain infection by upper case letters. (b) Representative confocal images of EHV1 IEP-positive plaques (green) in EREC monolayers, nuclei were detected with Hoechst (blue). The scale bar represents 50 µm.