Figure 7.

N-linked glycans, but not sialic acids play a role in EHV1 infection of EREC after basolateral inoculation. EREC were grown to confluency on transwells before enzymatic treatment with neuraminidase (1 h, 37 °C) or PNGase F (12 h, 37 °C) at either the apical or basolateral surface. Next, the same route was used for inoculation with EHV1 (MOI 1, 1 h, 37 °C) and cells were fixed in methanol 10 hpi before IEP staining. The total number of plaques was counted in five different fields of approximately 3∙10⁴ cells for each condition (left). Average plaque latitudes were measured on 10 individual plaques (right). Experiments were performed in triplicate on primary EREC of 3 different horses. Data are represented as means + SD and significant (P < 0.05) differences are indicated by different letters.