Fig. 4.

Anti-inflammatory effect of PNU-282987 and nicotine on LPS-induced mRNA expression of inflammatory mediators in IMCs. Rat mesothelial cells were treated with PNU-282987 (10 nM) and nicotine (10 nM) in the presence or absence of LPS (100 ng/ml) for 4 hr. PNU-282987 and nicotine were added 30 min before LPS application. (A–D) show results from IL-1β, TNF-α, iNOS and MCP-1, respectively. Each column shows mean ± SEM from four independent experiments (n=4−15); *P<0.05, **P<0.01 and ***P<0.001 vs. control values, and ^#P<0.05 and ^##P<0.01 vs. LPS values.