Table 1. Primer sets and expected product sizes for semi-quantitative RT-PCR.
| Target gene | Primer sequences |
Product sizes (bp) | |
|---|---|---|---|
| Forward | Reverse | ||
| GAPDH | TCCCTCAAGATTGTCAGCAA | AGATCCACAACGGATACATT | 308 |
| Mesothelin | TAGCCCCTGARGACATCCRSCAGTGG | CAACCGCCACATRACACTG | 251 |
| Keratin 5 | AAGGCCCAGTACGAGGACATT | GGTGTTCATGAGCTCCTGGTA | 351 |
| COX-2 | CTGTATCCCGCCCTCGTGGTG | ACTTGCGTTGATGGTGGCTGTCTT | 282 |
| iNOS | AAGRGAGTGYTGTTCCAGGT | CCACCAGCTTCTTCAAMGTG | 184 |
| IL-1β | TCCATGAGCTTTGTACAAGG | GGTGCTGATGACCAGTTGG | 246 |
| TNF-α | AAATGGGCTCCCTCTCATCA | AGCCTTGTCCCTTGAAGAGA | 248 |
| MCP-1 | CAACTCTCACTGAAGCCAGA | AAATGGATCTACATCTTGCA | 600 |
| α7nAChR | ATGGTGGCAAATGCCTAAG | CTCGGAAGCCAATGTAGAGC | 204 |
| α9nAChR | TCCTGGACCTACAATGGAAA | CTCCCAGAGAGACCTTCTCC | 295 |
| α10nAChR | AGATTGGAAGCGTCTGGCTA | TGCTCATCTGGCATTGAGTCTTA | 391 |