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Resolution assessment of the electron-counting cryo-EM structure of the human Ad5 virion. (A) The final resolution of the density map was estimated to be 3.2 Å by the criterion FSC = 0.143 (63). (B) High-resolution features of some protein side chains in the density map of cement protein VIII. Note that subtle differences among Val, Leu, and Ile, between Phe and Tyr, and between Lys and Arg are discernible.
