FIG 2.

Differential localization of nuclear and cytoplasmic LANA in KSHV-infected primary HOK and Vero cell cultures. (A) Primary HOK cultures induced to differentiate using high calcium (0.15 mM) for 24 h and (B) Vero monkey kidney epithelial cell cultures were infected with KSHV. At 24 hpi, the cell cultures were fixed and stained for LANA (green) using the LN53 antibody with TSA enhancement. Cell nuclei were labeled using TO-PRO-3 (blue). The arrowheads indicate cells with punctate nuclear LANA fluorescence, and the arrows indicate cells with diffuse LANA fluorescence in the cytoplasm. Scale bar, 20 μm.