TABLE 5.

Primers used for RT-qPCR detection of sncRNA in VZV-infected cells

sncRNA no. or universal primer or probe	Primer orientationa	Sequenceb
sncRNA
2	Reverse	GGTCGTATGCAAGAGCAGGGTCCGAGGTATCCATCGCACGCATCGCACTGCATACGACCTCCCGGGC
	Forward	GACTGATACCGTAGAACTACGC
9	Reverse	GGTCGTATGCAAGAGCAGGGTCCGAGGTATCCATCGCACGCATCGCACTGCATACGACCCACCCTGC
	Forward	CGCGGAAATGTCGGTTTCT
14	Reverse	GGTCGTATGCAAGAGCAGGGTCCGAGGTATCCATCGCACGCATCGCACTGCATACGACCGGTTACAC
	Forward	TAAAGTATGAGACTCGAGCCCTG
17	Reverse	GGTCGTATGCAAGAGCAGGGTCCGAGGTATCCATCGCACGCATCGCACTGCATACGACCAAGGCCAG
	Forward	TATGATACTGTCGACCCGAGAC
20	Reverse	GGTCGTATGCAAGAGCAGGGTCCGAGGTATCCATCGCACGCATCGCACTGCATACGACCGGCGCCAG
	Forward	GCGGGCAATGATTTCTGTCT
22	Reverse	GGTCGTATGCAAGAGCAGGGTCCGAGGTATCCATCGCACGCATCGCACTGCATACGACCTCCCCCGT
	Forward	TATATATAGACGGACCGGCCCCAA
23	Reverse	GGTCGTATGCAAGAGCAGGGTCCGAGGTATCCATCGCACGCATCGCACTGCATACGACCCACTGG
	Forward	AGACGTACGCCAATCGGATA
Universal reverse primer		GAGCAGGGTCCGAGGT
Universal probe		FAM–5′-TCCATCGCACGCATCGCACT-3′–BHQ-1

^aThe orientations of the specific reverse and forward primers are shown.

^bFAM, 6-carboxyfluorescein; BHQ-1, black hole quencher 1.