FIG 1.

Effects of ASFV infection on cellular and viral translation. (A) Polysome profile analysis. Vero cells were mock or ASFV infected (MOI = 5 PFU/cell), and samples were recovered at 14 hpi in the presence of cycloheximide (100 μg/ml). Polysomes were fractionated in sucrose gradients and examined in a polygraph; densitometry values were read at 260 nm and are represented as a function of time. O.D., optical density. (B) Quantification of cellular mRNA levels during ASFV infection. Samples from mock- or ASFV-infected (MOI = 5 PFU/cell) Vero cells were recovered at different times postinfection. After RNA extraction, cellular mRNA levels of representative genes were measured by RT-qPCR. RNA values are means and SD (n = 3). *, P < 0.03; **, P < 0.01; ***, P < 0.001.