FIG 4.

ASFV-DP interacts with the cellular translation machinery. (A) Analysis of the proteins enriched in the GST–ASFV-DP fraction after incubation with a sample from 18 hpi with ASFV. The numbers in the segments represent the quantities of proteins found in each category. (B) ASFV-DP cosediments with the ribosome. Mock- And 18-hpi infected samples were subjected to polysome profile analysis, fractions were recovered, and ASFV-DP and RPS6 presence was determined by Western blotting. (C) Analysis of the interaction of ASFV-DP and ribosomal protein RPL23a. Vero cells were infected with ASFV (MOI = 5 PFU/cell) and harvested at 18 hpi. Samples were incubated with anti-ASFV-DP or PS, and ASFV-DP and RPL23a presence was revealed by Western blotting (WB). The presence (+) or absence (−) of RNase A/T1 treatment is indicated.