TABLE 3.
Primers used in this work
| Name | Sequence |
|---|---|
| KpnI_promD250R_FW | GCGGGTACCTGAATATCTTGTGAACACAGC |
| BamHI_noSTOP_D250R_RV | GCGGGATCCGTGCTTATATCGTAAATAGTTTTTAATAAA |
| BamHI_g5Rwt_FW | GGGGGATCCATGGATACTGCCATGCAGCTT |
| EcoRI_g5Rwt_RV | GGGGAATTCCTACTAGTGCTTATATCGTAA |
| EcoRI_D250R_FW | GGGGAATTCATGGATACTGCCATGCAGCTT |
| BamHI_D250R_RV | GGGGGGGGATCCCTACTAGTGCTTATATCGTAA |
| D250R_mutREGG_FW | TCGGACCTTACCTGTGCCATAGGGGGGTTTGAAGAAGAAACCGGGATT |
| D250R_mutREGG_RV | AATCCCGGTTTCTTCTTCAAACCCCCCTATGGCACAGGTAAGGTCCGA |
| D250R_mutEEQQ_FW | TGTGCCATACGGGAGTTTGAACAACAAACCGGGATTACCCGCGAATAT |
| D250R_mutEEQQ_RV | ATATTCGCGGGTAATCCCGGTTTGTTGTTCAAACTCCCGTATGGCACA |
| KpnI_GFP_FW | GGGGGTAACATGGTGAGCAAGGGCGAGGAG |
| Nexo_GFPpD250R_FW | TCGAGCTCAAGCTTCGAATTCATGGATACTGCCATGCAGCTT |
| Nexo_GFPpD250R_RV | AAGCTGCATGGCAGTATCCATGAATTCGAAGCTTGAGCTCGA |
| BamHI_pD250R_RV | GGGGGGGGATCCCTACTAGTGCTTATATCGTAA |
| BamHI_pD250R_N-term_RV | GGGGGATCCCTACTAAATTTCCCATAGAAGTGT |
| Nexo_GFP_pD250R_Cterm_FW | AGCTCAAGCTTCGAATTCACCCGCGAATATTACCAG |
| Nexo_GFP_pD250R_Cterm_RV | AATCTGGTAATATTCGCGGGTGAATTCGAAGCTTGAGCTCGA |
| pD250RNtermCterm_FW | TCAGAAACACTTCTATGGGAAATTACCCGCGAATATTACCAGATTCTC |
| pD250RNtermCterm_RV | AATCTGGTAATATTCGCGGGTAATTTCCCATAGAAGTGTTTCC |
| 18S FW | GCAATTATTCCCCATGAACG |
| 18S RV | GGGACTTAATCAACGCAAGC |
| β-actin FW | AGGTCATCACCATTGGCAAC |
| β-actin RV | CGTGGATGCCACAGGACT |
| BIRC2 FW | GCTGACCCACCAATTATTCATTTTGG |
| BIRC2 RV | CACACACGTCAACTGTTGAAAGAGAGC |
| eIF4A1 FW | AATCCGCATCTTGGTGAAAC |
| eIF4A1 RV | CCCTCTCCACTGCCACAA |
| eIF4E FW | CAGCAGAGACGAAGTGACCTT |
| eIF4E RV | ACATTAACAACAGCGCCACA |
| p53 FW | GGCCCATCCTTACCATTATCA |
| p53 RV | AGCTGTTCCGTCCCAGTAGA |
| A238L FW | GCAGATCCGACTCAAAAAGACT |
| A238L RV | ACTCCATATTTCCTGTAAAGACTGC |
| A224L FW | AAGCACCCTTTACAGGTGCATGGC |
| A224L RV | TCCTATAATGCCCAAGGTTGCACGG |
| B646L FW | CCCGAGAACTCTCACAATATCC |
| B646L RV | CGTTGCGTCCGTAATAGGAG |