Positivity rates and ID50 titers of serum neutralization against tier 1A (A to D) and tier 1B and tier 2 (E to H) viruses measured in TZM-bl cells and serum titer (I) and peak granzyme B activity (J) of ADCC activity measured with B63531 gp120-coated target cells. Shaded bars represent group medians, and error bars represent interquartile ranges in panels B, D, F, and H. *, P < 0.05, and **, P < 0.01, for pairwise comparison (exact Wilcoxon rank sum test; values shown in Table 2). All differences are insignificant once adjusted for multiple comparison (Table 2).