FIG 10.

Location on Env of mutations that increase trimer stability or V2 neutralization. (A) Mutations that were found to increase PG9 neutralization or increase trimer stability are indicated as spheres on the crystal structure of JRFL envelope (PDB 5FYK). In the inset images, key residues at positions 165 and 169 (B) and position 315 (C) of gp120 are shown with residues nearby that may interact with them. Hydrogen bonds between neighboring amino acid residues, predicted using PyMOL, are shown using red dashed lines. I165 and R315 are shown in both inset images for orientation.