FIG 3.

Neutralization (MPN) of mutant Envs of HIV-1 ADA and Comb-mut by PG9. Substitutions were introduced into ADA pseudovirus and mutants were tested for sensitivity to PG9 neutralization at a constant concentration of 5 μg/ml. A subset of substitutions that showed a significant increase in MPN was then similarly tested in a Comb-mut Env background. Certain mutations that were predicted by structural analysis to potentially affect V2 and V3 antibody neutralization were also tested in Comb-mut but not ADA. Mutations that were not tested in a given background are labeled “nd” (not determined) or “ni” (not infectious). “N186-188” represents the triple mutant N186G/D187S/N188S. The red and green dashed lines indicate MPNs for wild-type ADA and Comb-mut, respectively. Red dots indicate mutants that show enhanced PG9 neutralization relative to wild-type ADA, green dots indicate enhanced PG9 neutralization of both ADA and Comb-mut. Each bar represents an average of biological triplicate values.