FIG 5.

Stability of Env correlates with enhanced MPN of PG9 and decreased neutralization by 447-52D. (A) Mutants of ADA were tested for thermostability and neutralization by PG9. T90 values are plotted against the percent neutralization at 5 μg/ml of PG9. A statistically significant relationship was observed between increased Env stability and an increase in PG9 neutralization. (B) Infectivity decay at 37°C of ADA, Comb-mut, and CM-LK. The results of a representative experiment performed in triplicate are shown, and the error bars represent the standard deviations. (C) Physical thermostability of Comb-mut and CM-LK Env trimers. BN-PAGE Western blot analysis of trimeric Envs solubilized from heat-treated virus shows that I165L/V169K do not change the trimer stability of Comb-mut.