FIG 9.

V3 crown neutralization correlates inversely with trimer thermostability and PG9 neutralization (MPN). (A) T90 values of mutants of ADA are plotted against the IC₅₀ of V3 antibody 447-52D. A statistically significant relationship is seen between increased Env stability and a decrease in 447-52D neutralization. (B) The IC₅₀ of 447-52D is plotted against the MPN at 5 μg/ml of PG9. Mutants in the V2 domain (between amino acids 160 and 190) that might directly affect PG9 binding were removed from the analysis. (C) Neutralization of Comb-mut, CM-LK, and CM-LK R315Q pseudovirions by a panel of antibodies shows that R315Q abrogates neutralization by V3 crown antibody 447-52D (middle panel) without altering the neutralization properties of PG9 (top panel) and VRC01 (bottom panel). Data shown are from a representative experiment performed in triplicate, and error bars represent the standard deviations from the mean.