TABLE 1.
Plating efficiency of temperature-sensitive and lethal mutants
| Mutanta | Efficiency at indicated type of temp (°C)b |
Efficiency under restrictive conditionc with exogenous gene expression (temp, °C) |
||
|---|---|---|---|---|
| Permissive | Restrictive | F gene | B gene | |
| ts(F)F67N | 1.0 (33) | 10−6 (42) | 1.1 (42) | 0.3 (42) |
| ts(F)F67W | 1.0 (33) | <10−4 (42) | 1.0 (42) | <10−4 (42) |
| l(F)F67D | 10−4 (37) | 1.0 (37) | 1.0 (37) | |
| l(F)F67E | 10−4 (37) | 1.0 (37) | 10−4 (37) | |
| l(F)F67K | 10−4 (37) | 1.0 (37) | 10−4 (37) | |
| l(F)F67R | 10−4 (37) | 1.0 (37) | 10−4 (37) | |
| ts(F)Y134D | 1.0 (33) | 10−3 (42) | 0.9 (42) | 0.1 (42) |
| ts(F)Y134E | 1.0 (28) | <10−6 (37) | 0.5 (37) | <10−6 (37) |
| ts(F)Y134L | 1.0 (33) | 10− (42) | 1.0 (42) | 0.6 (42) |
| ts(F)Y134Q | 1.0 (33) | 10−5 (42) | 0.9 (42) | 0.9 (42) |
| ts(F)Y134T | 1.0 (33) | 10−5 (42) | 0.7 (42) | 0.7 (42) |
| ts(F)Y134V | 1.0 (33) | 10−5 (42) | 1.1 (42) | 1.0 (42) |
| ts(F)Y134R | 1.0 (33) | 10−3 (37) | 1.0 (37) | 0.9 (37) |
| ts(F)F135C | 1.0 (33) | <10−4 (42) | 1.1 (42) | 0.6 (42) |
| ts(F)F135H | 1.0 (33) | <10−5 (37) | 1.0 (37) | 10−5 (37) |
| ts(F)F135I | 1.0 (33) | <10−6 (42) | 1.0 (42) | 0.7 (42) |
| ts(F)F135W | 1.0 (28) | 10−4 (33) | 1.0 (33) | <10−3 (33) |
| l(F)F135K | 10−4 (37) | 1.0 (37) | 10−4 (37) | |
a
Mutant names reflect the phenotype (ts, temperature sensitive; l, lethal) and conferred substitution. The letters in parentheses indicate the genes. Thus, the designation ts(F)F67N means that an F→N at position 67 within gene F confers a temperature-sensitive phenotype.
b
For ts mutants, the restrictive and permissive temperatures are given in parentheses. For lethal mutants, all experiments were conducted at 37°C. The permissive condition includes the exogenous expression of a cloned F gene.
c
For ts mutants, experiments involving the exogenous expression of either the cloned B or F gene were conducted at the restrictive temperature.