FIG 2.
Biochemical characterization of mutated rabbit rec-PrP-containing substrates showed similar amounts of rec-PrP present. The Western blot shows similar amounts of the different recPMCA substrates based on wild-type rabbit rec-PrPs (wt rPrP) with specific mouse substitutions. Note the presence of a single band of approximately 20 kDa in the absence of protease K (PK) treatment. Wild-type rabbit and mouse rec-PrP-containing substrates are also included. The lower signal for the wild-type mouse rec-PrP (mouse rPrP) can be explained by a lower affinity of the monoclonal antibody SAF84 (1:400) which was used to develop the membranes. Rabbit PrPC (rabbit normal brain homogenate) was run in a separate gel, and the image is shown divided from the other gel by a vertical gray line.