FIG 2.

Overexpression of miR-424 suppresses HPV genome amplification upon keratinocyte differentiation. HPV31-positive CIN612 cells stably expressing a scrambled vector control or miR-424 were incubated for 72 h of differentiation in high-calcium media. (A) RT-PCR analysis of miR-424 in miR-424-overexpressed CIN612 cells grown in an undifferentiated monolayer culture. (B) Southern blot analysis of HPV31 episomes in CIN612 cells stably expressing miR-424 with differentiation in high-calcium media for the indicated times (in hours). (C) The levels of HPV episome shown in Fig. 2B were quantified using the ImageJ software and are presented graphically. (D) The growth rate of miR424-expressing cells was compared to the one of vector control cells. All results are representative of observations from two or more independent experiments.