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. 2017 Oct 24;11(12):1687–1697. doi: 10.1002/1878-0261.12113

Figure 4.

Figure 4

(A) CellSearch® was used to enrich CTCs from a SCLC patient blood sample and DEPArray™ was used to isolate CTCs from a portion of the enriched sample prior to storage in glycerol at −20 °C. All isolated cells were subjected to WGA plus GII‐PCR and data presented using the same format as in Fig. 2 panel A. (B) An aliquot of the same CellSearch®‐enriched cells described in panel A was stored in glycerol at −20 °C for 17 months prior to CTC isolation by DEPArray™. All isolated cells were subjected to WGA plus GII‐PCR and data presented using the same format as in Fig. 2 panel A. (C) A comparison of the GII from cells enriched by CellSearch® and DEPArray™ isolated prior to glycerol storage (0 months), and stored at −20 °C for 17 months in glycerol prior to DEPArray™ isolation. No statistically significant difference in the GII values was found following long‐term storage (Mann–Whitney test; P = 0.820; error bars show SD).