Figure 6.

Upregulated RNF44 and downregulated AMPK‐α1 sensitize BR xenograft tumors to ADI‐PEG20 treatment. (A,B) Corresponding to our previous study (Li et al., 2016), ADI‐PEG20 significantly reduced BR tumor sizes by inducing cell apoptosis. Female nude mice were inoculated subcutaneously with 1 × 10⁶ melanoma cells. When tumor volume reached 100 mm³, tumor‐bearing mice received treatment of ADI‐PEG20 (100 IU·kg⁻¹) twice per week. On day 32, single‐cell suspensions were prepared from tumor tissues and then were labeled with Annexin V and then analyzed by FACS. (C) (D) AMPK‐α1 and RNF44 expressions were detected by IHC staining, and the data were presented as H‐scores (scale bar = 100 μm) (n = 5, **P < 0.01, and ***P < 0.005).